A human mannose-binding protein is an acute-phase reactant that shares sequence homology with other vertebrate lectins.

A human mannose-binding protein is an acute-phase reactant that shares sequence homology with other vertebrate lectins [published erratum appears in J Exp Med 1991 Sep 1;174(3):753]

Mannose-binding proteins have been isolated from the liver of rats and humans and subsequently been found in the serum of rats, rabbits, and humans. We report the isolation of cDNA clones isolated from a human liver cDNA library that encodes a human mannose-binding protein. The primary structure has three domains: (a) an NH2-terminal cysteine-rich segment of 19 amino acids which appears to be i...

Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin.

Eukaryotic chromosome segregation depends on the mitotic spindle apparatus, a bipolar array of microtubules nucleated from centrosomes. Centrosomal microtubule nucleation requires attachment of gamma-tubulin ring complexes to a salt-insoluble centrosomal core, but the factor(s) underlying this attachment remains unknown. In budding yeast, this attachment is provided by the coiled-coil protein S...

Identification of a lipid A binding site in the acute phase reactant lipopolysaccharide binding protein.

Lipopolysaccharide (LPS) binding protein (LBP), a recently discovered 60-kDa acute phase protein, is present in the acute phase serum of many species including human, rabbits, mice, and rats. Using either highly purified LBP from acute phase rabbit serum or unfractionated acute phase rabbit serum as a source of LBP, we examined the binding of LBP to LPS immobilized on plastic microtiter plates ...

Is an RNA-Binding Protein That Is A Human Sequence Homologue of Staufen

The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factors.

We have shown previously that the PRP2(RNA2) gene product of the yeast, Saccharomyces cerevisiae is required to convert the pre-mRNA to linear exon 1 and lariat intron-exon 2 in the spliceosome (1). Here we report the nucleotide sequence of a 3177 bp EcoRI/BamHI DNA fragment within which the PRP2 gene is located (2 —4; Figure 1). An open reading frame encoding a protein of 876 amino acids is fo...